Abstract Surge arresters are at the forefront of protection against voltage surges. The loss of the protective effect of surge arresters imposes accidents and unplanned shutdowns on the power system. It directly affects the interruption of power supplies and imposes fines on service providers. Monitoring and diagnostics in the lightning rod field require studies and work that present the degradation behaviour of surge arresters to help develop and improve existing techniques. This paper presents the development of a laboratory with a low-energy, mobile current waveform impulse generator. It allows for conducting aging tests by applying discharges to low-voltage zinc oxide surge arresters used in the energy distribution sector. Samples of surge arresters were subjected to the discharges produced by the developed generator, and they exhibited degradation, thus showing asymmetry between the positive and negative half-cycles of leakage current.

Abstract The presented development is an intelligent diagnostic system for transformers that studied machine learning techniques to determine the operational status of these transformers. The study of these techniques is initiated by observing the quantities that define the operational behavior of large transformers, aiming to identify anomalies in their operation from data from sensors that equipment it in the functioning environment. This large power transformer has a theoretical service life of above 20 years and a low failure rate. Thus, obtaining failure values, which have their evolution monitored for large transformers, is almost nil. Therefore, a supervised machine training methodology to diagnose these cases is practically unfeasible. The study carried out with several traditional intelligent techniques can verify this. Several supervised methods (Closest Neighbor K-th Neighbor, Support Vector Machine, Radial Base Function, Decision Trees, Random Forest, Neural Network, AdaBoost, Gaussian Naive Bayes, and Quadratic Discriminant Analysis) were studied.

Abstract We evaluate how the concentrations of inorganic nutrients and chlorophyll a vary in a heterogeneous area (Equatorial SW Atlantic), covering a gradient from stations closer to the coast to others more distant associated or not with turbid-zone reefs. Vertical temperature (27.9 ± 0.10 °C; mean ± standard deviation) and salinity (36.2 ± 0.14) profles showed that the water column is well mixed (0-30 m depth). The oligotrophic condition was marked by low concentrations of phosphate (0.30 ± 0.22 µM) and dissolved inorganic nitrogen (0.64 ± 0.74 µM). Moreover, dissolved reactive silicon (DSi) was low in most samples (< 2.0 µM), but higher (>10 µM) in nearshore stations, probably related to continental runoff and/or resuspension of the bottom sediments. The pelagic phytoplankton biomass indicated that chlorophyll a (0.25 ± 0.08 µg L-1) was low, positively correlated with light and negatively correlated with nutrients, indicating possible phytoplankton uptake. Chlorophyll a concentrations were lower in stations closer to the coast and higher in some stations near the reefs, indicating that the latter could be more prone to phytoplankton development and depletion of nutrients, especially DSi. Therefore, although oligotrophy is present along this coast, we found some unexpected heterogeneity of nutrient and chlorophyll a distributions, which were probably infuenced by benthic-pelagic coupling due to the presence of extensive reefs, sponge gardens (18-30 m depth), and the proximity to the coast. These results highlight the importance of understanding the heterogeneity of ocean productivity, especially in lesser known low-latitude areas, which showed distinct nutrient and chlorophyll a levels related to the occurrence of tropical reefs that are capable of supporting important fsh stocks and unique biological communities.

Resumo O uso de polímeros hidrorretentores surge como uma alternativa para disponibilizar água de forma adequada na fase de implantação de povoamentos florestais, além de reter nutrientes necessários para o estabelecimento e desenvolvimento destes. No presente trabalho, objetivou-se avaliar o efeito do polímero hidrorretentor e a influência de diferentes doses de fertilização mineral no crescimento, teor e acúmulo de nutrientes do híbrido clonal de Eucalyptus urophylla x Eucalyptus grandis, na fase de implantação. O experimento foi realizado em delineamento de blocos casualizados completos, em esquema fatorial 2 x 5, sendo duas doses do polímero (0 e 1 g planta-1) e cinco combinações de fertilização mineral (N0P0K0, N0P1K0, N1P0K1, N1P1K1 e N2P2K2, correspondendo à dose de N (N0 = 0, N1 = 62 e N2 = 124 g do fertilizante por planta), P (P0 = 0, P1 = 75 e P2 = 150 g do fertilizante por planta) e K (K0 = 0, K1 = 62 e K2 = 124 g do fertilizante planta)), em três repetições com cinco plantas por parcela. As avaliações foram realizadas aos 30, 60, 120 e 150 dias pós-plantio, quando foram mensurados a altura total e o diâmetro do colo, além da análise química nutricional, aos 150 dias. A partir dos dados obtidos, observou-se que não houve interação entre o efeito do polímero e doses de fertilização mineral. Para o diâmetro do colo, o uso do polímero proporcionou maior crescimento às mudas somente nos 30 primeiros dias de avaliação, e para altura houve maior crescimento nas avaliações aos 30, 60 e 120 dias de plantio. Os tratamentos contendo fósforo (18% de P2O5) obtiveram maior incremento em diâmetro em relação aos demais nos primeiros 60 dias pós-plantio. Nas outras avaliações, a maior dose de fertilizante (N2P2K2) foi responsável pelo maior crescimento em diâmetro e altura. A maior dose de fertilização mineral promoveu maior acúmulo de NPK na parte aérea das plantas (caule, ramos e folhas). Portanto, o polímero proporcionou maior crescimento às mudas na fase inicial de desenvolvimento, e o fósforo se mostrou essencial para o crescimento em diâmetro e altura na fase de implantação do híbrido utilizado.

Abstract The use of water retaining polymer appears as an alternative to provide adequate water the phase of implantation the forest stands, further retaining nutrients necessary for the establishment and development of these. The objective of this study was to evaluate the effect of the water retaining polymer and influence of the different forms of mineral fertilization at growth, content and nutrient accumulation of a clonal hybrid of Eucalyptus urophylla x Eucalyptus grandis in the implantation phase. The experiment was carried out in a randomized complete block design at 2 x 5 factorial scheme, with two polymer doses (0 and 1 g plant-1) and five mineral fertilization combinations (N0P0K0, N0P1K0, N1P0K1, N1P1K1 e N2P2K2, corresponding to the dose of N (N0 = 0, N1 = 62 e N2 = 124 g of fertilizer per plant), P (P0 = 0, P1 = 75 e P2 = 150 g of fertilizer per plant) e K (K0 = 0, K1 = 62 e K2 = 124 g of fertilizer per plant)) in three replications with five plants per plot. The valuates were performed at 30, 60, 120 and 150 days post-planting, when total height and root collar diameter were measured, as well as nutritional chemical analysis, at 150 days. From the obtained data, observed that there was no interaction between the effect of the polymer and doses of mineral fertilization. For the root collar diameter use of the polymer only provided bigger growth seedlings at 30 days the evaluate, and for the height, there was greater growth in the valuates in the 30, 60 and 120 days of planting. The phosphorus treatments were obtained a bigger increment to the diameter in relation for the others 60 days after planting. These the other evaluations of higher fertilizer dosage (N2P2K2) was responsible for the greater growth in diameter and height. The higher dosage of mineral fertilization promoted greater accumulation at NPK in the aerial part of the plants (stem, branches e leaves). Therefore, the polymer gave a higher growth seedling in the initial phase development, and the phosphorus was shown to be essential for the growth in diameter and height in the phase of implantation of the hybrid used.

Este estudo teve como objetivo avaliar a atividade antifúngica de extratos de M. oleifera frente a fungos isolados de camarões, cultivados em água doce, e testar a toxicidade dos extratos em larvas de Macrobrachium amazonicum. Os extratos etanólicos de vagens, sementes, folhas, caules e flores e o extrato clorofórmico de flores de M. oleifera foram testados contra 14 cepas de Candida spp. e 10 cepas de Hortaea werneckii isolados da água de cultivo e do trato digestório de M. amazonicum. A atividade antifúngica foi determinada por microdiluição, com base nos documentos M27-A3 e M38-A2 do CLSI. A toxicidade foi avaliada por exposição das larvas de M. amazonicum a concentrações entre 10-1000 mg mL-1 dos extratos, realizando contagem de larvas mortas (CL50), após 24 horas. Os melhores resultados foram verificados com o extrato clorofórmico de flores, agindo frente a todas as cepas testadas, com concentrações inibitórias mínimas variando entre 0,019-2,5 mg mL-1. O extrato etanólico de folhas, flores e sementes agiu ante 22/24, 21/24 e 20/24 cepas, respectivamente. O extrato de vagens foi eficaz contra cepas de Candida spp. (14/24) e o extrato de caule apenas contra quatro cepas de H. werneckii (4/24). Os extratos de sementes, flores (fração clorofórmica), caules e folhas apresentaram baixa ou nenhuma toxicidade, enquanto que extratos de vagens e flores (fração etanólica) apresentaram toxicidade moderada. Assim, observou-se atividade antifúngica dos extratos em Candida spp . e H. werneckii com uma ampla margem de segurança para as larvas de M. amazonicum, demonstrando ser promissor para o manejo sustentável dos efluentes do cultivo de M. amazonicum

This study aimed to evaluate the antifungal activity of M. oleifera extracts against fungi isolated from farmed prawns and test the toxicity of the extracts on larvae of Macrobrachium amazonicum. The ethanol extracts of pods, seeds, leaves, stems and flowers and chloroform extract of flowers of M. oleifera were tested against 14 strains of Candida spp. and 10 strains of Hortaea werneckii isolated from farming water and the digestive tract of M. amazonicum. Antifungal activity was determined by microdilution, based on the M27-A3 and M38-A2 CLSI documents. Toxicity was evaluated by exposing larvae of M. amazonicum at concentrations between 10-1000mg mL-1, counting dead larvae (CL50) after 24 hours. The best results were verified with the chloroform extract of flowers, acting against all tested strains, with MICs ranging from 0.019 to 2.5 mg mL-1. Ethanol extracts of leaves, flowers and seeds acted against 22/24, 21/24 and 20/24 strains, respectively. The extract of pods was only effective against strains of Candida spp. (14/24) and extract of stem only against four strains of H. werneckii (4/24). Extracts of seeds, flowers (chloroform fraction), stems and leaves showed low or no toxicity, whereas extracts of pods and flowers (ethanol fraction) showed moderate toxicity. Thus, the antifungal activity of these extracts agaisnt Candida spp. and H. werneckii was observed, a wide margin of safety for larvae of M. amazonicum, demonstrating to be promising for the sustainable management of effluents from M. amazonicum farming

Este estudo teve como objetivo avaliar o papel do camarão Macrobrachium amazonicum como carreador de Candida spp. do ambiente aquático, por meio da análise da correlação entre Candida spp. isoladas desse camarão e do seu ambiente (água de superfície e sedimento) através das técnicas de M13-PCR fingerprinting e RAPD-PCR. Para tanto, 27 cepas de Candida spp. foram avaliadas. Essas cepas foram recuperadas a partir do trato gastrointestinal de M. amazonicum adultos (7/27), oriundos da lagoa do Catú, Ceará, Brasil, e do meio aquático (águas superficiais e sedimentos) desse lago (20/27). A comparação molecular entre as cepas de camarões e o ambiente aquático foi conduzida por M13-PCR fingerprinting e RAPD-PCR, utilizando os iniciadores M13 e OPQ16, respectivamente. A análise molecular revelou semelhanças entre os padrões de bandas de oito isolados de Candida com o iniciador M13 e 11 isolados com o primer OPQ16, indicando que elas estão presentes no trato digestivo de M. amazonicum e no ambiente aquático, onde esses camarões habitam. Portanto, essa espécie de camarão pode ser usada como sentinela para monitoramento ambiental através da recuperação de Candida spp. do ambiente aquático, a partir do seu trato gastrointestinal

This study aimed to evaluate the role of the Amazon River prawn, Macrobrachium amazonicum, as carrier of Candida spp., by analyzing the correlation between Candida spp. from these prawns and their environment (surface water and sediment), through M13-PCR fingerprinting and RAPD-PCR. For this purpose, 27 strains of Candida spp. were evaluated. These strains were recovered from the gastrointestinal tract of adult M. amazonicum (7/27) from Catú Lake, Ceará State, Brazil and from the aquatic environment (surface water and sediment) of this lake (20/27). Molecular comparison between the strains from prawns and the aquatic environment was conducted by M13-PCR fingerprinting and RAPD-PCR, utilizing the primers M13 and OPQ16, respectively. The molecular analysis revealed similarities between the band patterns of eight Candida isolates with the primer M13 and 11 isolates with the primer OPQ16, indicating that the same strains are present in the digestive tract of M. amazonicum and in the aquatic environment where these prawns inhabit. Therefore, these prawns can be used as sentinels for environmental monitoring through the recovery of Candida spp. from the aquatic environment in their gastrointestinal tract

O aumento da incidência das infecções fúngicas, bem como o registro crescente de resistência e falha terapêutica, têm impulsionado a realização de estudos de prospecção de fitoquímicos com propriedades antifúngicas. Diante do exposto, o presente estudo investigou o potencial antifúngico de extratos de Baccharis ligustrina, B. schultzii, Croton jacobinensis, Licania rigida, Moringa oleifera, Vernonia sp. e V. brasiliana, e de óleos essenciais de Lippia alba (Quimiotipos 1, 2, 3 e 4) e Ocimum gratissimum. Inicialmente, foi realizada uma avaliação qualitativa da atividade antifúngica de cada amostra por meio do método de difusão em ágar, frente a cepas de Candida albicans e Microsporum canis, mostrando que apenas os extratos de M. oleifera (MLF-C) e Vernonia sp. (TVS-H) apresentaram atividade frente a C. albicans e M. canis, com halos de inibição =10mm. Também foram determinadas a concentração inibitória mínima (CIM), frente a 12 cepas de C. albicans e M. canis, e a toxicidade aguda de MLF-C e TVS-H, através de protocolos do Clinical and Laboratory Standards Institute (CLSI) e ensaio com Artemia sp., respectivamente. A CIM (80%) de MLF-C e TVS-H variou de 0,156 a 2,5mg mL-1 frente C. albicans e de 0,039 a 1,25 e 0,039 a 0,625mg mL-1 para M. canis, respectivamente. A CIM (100%) de MLF-C e TVS-H variou de 0,625 a 2,5mg mL-1 frente C. albicans é de 0,039 a 2,5 e 0,078 a 1,25mg mL-1 para M. canis, respectivamente. As doses letais (DL50) para o MLF-C e TVS-H foram de 201,09 e 204,17mg mL-1, respectivamente, sendo, portanto, demonstrada a baixa toxicidade desses extratos. Os extratos de M. oileifera e Vernonia sp. apresentaram atividade antifúngica frente cepas de C. albicans e M. canis, abrindo a perspectiva de estudos para caracterização dos seus componentes bioativos.

The increase in the incidence of fungal infections and the frequent report of resistance and therapeutic failure has promoted the performance of phytochemical screening for compounds with antifungal properties. Based on this, the present study investigated the antifungal potential of extracts of Baccharis ligustrina, B. schultzii, Croton jacobinensis, Licania rigida, Moringa oleifera, Vernonia sp. and V. brasiliana and of essential oils of Lippia alba (Chemotypes 1, 2, 3 and 4) and Ocimum gratissimum. Initially, a qualitative evaluation of the antifungal activity of each vegetal sample against strains of Candida albicans and Microsporum canis, through the agar diffusion method, was performed. Extracts of M. oleifera (MLF-C) and Vernonia sp. (TVS-H) presented activity against C. albicans and M. canis with inhibition halos =10mm. Then, minimum inhibitory concentrations (MICs) for MLF-C and TVS-H against 12 strains of C. albicans and M. canis were determined through the methodology established by the Clinical and Laboratory Standards Institute (CLSI), and acute toxicity tests against Artemia sp. were performed for both extracts. MICs (80%) for MLF-C and TVS-H varied from 0.156 to 2.5mg mL-1 against C. albicans and from 0.039 to 1.25mg mL-1 and 0.039 to 0.625mg mL-1 against M. canis, respectively. MICs (100%) for MLF-C and TVS-H varied from 0.625 to 2.5mg mL-1 for C. albicans and from 0.039 to 2.5mg mL-1 and 0.078 to 1.25mg mL-1 against M. canis, respectively. Lethal doses (DL50) of MLF-C and TVS-H were 201.09 and 204.17mg mL-1, respectively, being, therefore, demonstrated the low toxicity of these extracts. M. oleifera and Vernonia sp. extracts presented in vitro antifungal activity against C. albicans and M. canis, creating perspectives for the development of studies on the characterizations of their bioactive components.